PAF-induced MAPK activation is inhibited by wortmannin in neutrophils and macrophages

I Ferby; I Waga; K Kume; C Sakanaka; T Shimizu

doi:10.1007/978-1-4899-0179-8_51

PAF-induced MAPK activation is inhibited by wortmannin in neutrophils and macrophages

Adv Exp Med Biol. 1996:416:321-6. doi: 10.1007/978-1-4899-0179-8_51.

Authors

I Ferby¹, I Waga, K Kume, C Sakanaka, T Shimizu

Affiliation

¹ Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.

PMID: 9131167
DOI: 10.1007/978-1-4899-0179-8_51

Abstract

In the present study we examined the mechanism by which PAF activates MAPK in native cells such as guinea-pig neutrophils and P388D1 macrophage-like cells. We found that PAF activates MAPK through two distinct pathways. One calcium-dependent pathway that likely involves cPKC, and another calcium-independent but wortmannin-sensitive pathway. Using molecular biological methods we are presently examining whether hetrodimeric (p85/p110) type PI 3-kinase is the actual target of wortmannin involved in PAF mediated activation of MAPK.

MeSH terms

Androstadienes / pharmacology*
Animals
Calcium / physiology
Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
Cyclic AMP-Dependent Protein Kinases / metabolism*
Enzyme Activation / drug effects
Enzyme Inhibitors / pharmacology*
Guinea Pigs
In Vitro Techniques
Macrophages / drug effects
Macrophages / enzymology*
Neutrophils / drug effects
Neutrophils / enzymology*
Platelet Activating Factor / antagonists & inhibitors*
Platelet Activating Factor / physiology*
Wortmannin

Substances

Androstadienes
Enzyme Inhibitors
Platelet Activating Factor
Cyclic AMP-Dependent Protein Kinases
Calcium
Wortmannin