Abstract
We have produced novel bispecific antibodies by fusing the DNA encoding a single chain antibody (ScFv) after the C terminus (CH3-ScFv) or after the hinge (Hinge-ScFv) with an antibody of a different specificity. The fusion protein is expressed by gene transfection in the context of a murine variable region. Transfectomas secrete a homogeneous population of the recombinant antibody with two different specificities, one at the N terminus (anti-dextran) and one at the C terminus (anti-dansyl). The CH3-ScFv antibody, which maintains the constant region of human IgG3, has some of the associated effector functions such as long half-life and Fc receptor binding. The Hinge-ScFv antibody which lacks the CH2 and CH3 domains has no known effector functions.
MeSH terms
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Animals
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Antibodies, Bispecific / biosynthesis*
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Antibodies, Bispecific / chemistry*
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Antibodies, Bispecific / isolation & purification
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Antibody Affinity
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Chromatography, Gel
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Dansyl Compounds
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Drug Design
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Enzyme-Linked Immunosorbent Assay
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Humans
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Immunoglobulin Constant Regions / biosynthesis*
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Immunoglobulin Constant Regions / chemistry
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Immunoglobulin G / biosynthesis*
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Immunoglobulin G / chemistry
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Immunoglobulin Heavy Chains
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Immunoglobulin Variable Region
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Kinetics
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Ligands
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Mice
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Multiple Myeloma
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Recombinant Fusion Proteins / biosynthesis*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / isolation & purification
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Transfection
Substances
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Antibodies, Bispecific
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Dansyl Compounds
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Immunoglobulin Constant Regions
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Immunoglobulin G
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Immunoglobulin Heavy Chains
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Immunoglobulin Variable Region
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Ligands
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Recombinant Fusion Proteins