Abstract
Introduction: It has been reported that long noncoding RNAs (lncRNAs) are crucial regulators in progression of human cancers, including colorectal cancer (CRC). However, the function of lncRNA ARAP1 antisense RNA 1 (ARAP1-AS1) in CRC remains unclear. Aim: The aim of this study was to investigate the function and molecular mechanism of lncRNA ARAP1-AS1 in CRC. Results: ARAP1-AS1 was highly expressed in CRC tissues and cell lines. ARAP1-AS1 knockdown suppressed cell migration, invasion, and epithelial-mesenchymal transition (EMT). YY1 transcription factor (YY1) enhanced the transcription activity of ARAP1-AS1. The YY1/ARAP1-AS1 axis promoted CRC cell migration and invasion. YY1/ARAP1-AS1 could regulate the Wnt/β-catenin signaling pathway. Conclusions: This study revealed that YY1-induced upregulation of ARAP1-AS1 promoted cell migration, invasion, and EMT process in CRC through the Wnt/β-catenin signaling pathway.
Keywords:
Wnt/β-catenin signaling pathway; YY1; colorectal cancer; lncRNA ARAP1-AS1; migration.
MeSH terms
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Apoptosis
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism
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Carrier Proteins / antagonists & inhibitors*
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Carrier Proteins / genetics
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Cell Movement*
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Cell Proliferation
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Colorectal Neoplasms / genetics
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Colorectal Neoplasms / metabolism
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Colorectal Neoplasms / pathology*
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Epithelial-Mesenchymal Transition
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Female
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GTPase-Activating Proteins / antagonists & inhibitors*
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GTPase-Activating Proteins / genetics
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Gene Expression Regulation, Neoplastic
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Humans
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Lymphatic Metastasis
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Male
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Middle Aged
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Neoplasm Invasiveness
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Prognosis
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RNA, Long Noncoding / genetics
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RNA, Long Noncoding / metabolism*
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Tumor Cells, Cultured
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Wnt Proteins / genetics
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Wnt Proteins / metabolism*
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Wnt Signaling Pathway
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YY1 Transcription Factor / genetics
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YY1 Transcription Factor / metabolism*
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beta Catenin / genetics
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beta Catenin / metabolism*
Substances
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ARAP1 protein, human
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Biomarkers, Tumor
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CTNNB1 protein, human
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Carrier Proteins
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GTPase-Activating Proteins
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RNA, Long Noncoding
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Wnt Proteins
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YY1 Transcription Factor
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YY1 protein, human
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beta Catenin