miR-1306-3p targets FBXL5 to promote metastasis of hepatocellular carcinoma through suppressing snail degradation

Zhi-Jiang He; Wen Li; Hua Chen; Jian Wen; Yan-Feng Gao; Yun-Jun Liu

doi:10.1016/j.bbrc.2018.09.059

miR-1306-3p targets FBXL5 to promote metastasis of hepatocellular carcinoma through suppressing snail degradation

Biochem Biophys Res Commun. 2018 Oct 12;504(4):820-826. doi: 10.1016/j.bbrc.2018.09.059. Epub 2018 Sep 12.

Authors

Zhi-Jiang He¹, Wen Li², Hua Chen², Jian Wen², Yan-Feng Gao², Yun-Jun Liu²

Affiliations

¹ Department of Oncology 2, The People's Hospital, Maoming, 525000, Guangdong, PR China. Electronic address: hezj8171@163.com.
² Department of Oncology 2, The People's Hospital, Maoming, 525000, Guangdong, PR China.

PMID: 30219228
DOI: 10.1016/j.bbrc.2018.09.059

Abstract

This study aimed to elucidate the effect of miR-1306-3p on metastasis of hepatocellular carcinoma (HCC) and potential mechanism involved. miR-1306-3p promoted migration and invasion of HCC in vivo and in vitro. Moreover, miR-1306-3p inhibited snail to enhance its expression via directly targeting FBXL5, thus inducing the epithelial-mesenchymal transition (EMT) in HCC. Intriguingly, miR-1306-3p expression was transcriptionally enhanced by FoxM1. Consistently, miR-1306-3p was upregulated in HCC compared with paracarcinoma and correlated with poor prognosis of HCC patients. Our researches suggest that miR-1306-3p is a tumor enhancer in regulating of HCC metastasis, and miR-1306-3p may be clinically utilized as a factor for the clinical diagnosis and prognosis of HCC.

Keywords: EMT; FBXL5; Hepatocellular carcinoma; miR-1306–3p; microRNAs.

MeSH terms

Animals
Biomarkers, Tumor / genetics
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / mortality
Carcinoma, Hepatocellular / pathology*
Cell Line, Tumor
Epithelial-Mesenchymal Transition / genetics
F-Box Proteins / genetics*
F-Box Proteins / metabolism
Forkhead Box Protein M1 / genetics
Forkhead Box Protein M1 / metabolism
Gene Expression Regulation, Neoplastic
Humans
Liver Neoplasms / genetics
Liver Neoplasms / metabolism
Liver Neoplasms / mortality
Liver Neoplasms / pathology*
Male
Mice, Inbred BALB C
MicroRNAs / genetics
MicroRNAs / metabolism*
Oncogenes
Prognosis
Proteolysis
Snail Family Transcription Factors / genetics
Snail Family Transcription Factors / metabolism*
Ubiquitin-Protein Ligase Complexes / genetics*
Ubiquitin-Protein Ligase Complexes / metabolism
Xenograft Model Antitumor Assays

Substances

Biomarkers, Tumor
F-Box Proteins
FBXL5 protein, human
FOXM1 protein, human
Forkhead Box Protein M1
MIRN1306 microRNA, human
MicroRNAs
SNAI1 protein, human
Snail Family Transcription Factors
Ubiquitin-Protein Ligase Complexes