Abstract
Lung cancer is the leading cause of cancer mortality worldwide and non-small-cell lung cancer (NSCLC) is the most common type. Marine plants provide rich resources for anticancer drug discovery. Fucoxanthin (FX), a Laminaria japonica extract, has attracted great research interest for its antitumor activities. Accumulating evidence suggests anti-proliferative effects of FX on many cancer cell lines including NSCLCs, but the detailed mechanisms remain unclear. In the present investigation, we confirmed molecular mechanisms and in vivo anti-lung cancer effect of FX at the first time. Flow cytometry, real-time PCR, western blotting and immunohistochemistry revealed that FX arrested cell cycle and induced apoptosis by modulating expression of p53, p21, Fas, PUMA, Bcl-2 and caspase-3/8. These results show that FX is a potent marine drug for human non-small-cell lung cancer treatment.
Keywords:
apoptosis; cell cycle arrest; fucoxanthin; non-small-cell lung cancer.
MeSH terms
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A549 Cells
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Animals
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Antineoplastic Agents, Phytogenic / therapeutic use*
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Apoptosis / drug effects
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Apoptosis Regulatory Proteins / metabolism
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Carcinoma, Non-Small-Cell Lung / drug therapy*
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Cell Cycle / drug effects
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Cell Division / drug effects
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Female
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Flow Cytometry
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Humans
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Immunohistochemistry
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Laminaria / chemistry*
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Lung Neoplasms / drug therapy*
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use*
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Real-Time Polymerase Chain Reaction
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Tumor Suppressor Proteins / metabolism
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Xanthophylls / isolation & purification
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Xanthophylls / pharmacology
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Xanthophylls / therapeutic use*
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Xenograft Model Antitumor Assays
Substances
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Antineoplastic Agents, Phytogenic
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Apoptosis Regulatory Proteins
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Plant Extracts
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Tumor Suppressor Proteins
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Xanthophylls
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fucoxanthin