Abstract
TWIST1 is a highly conserved basic helix-loop-helix transcription factor that promotes epithelial-mesenchymal transition (EMT). Its misregulation has been observed in various types of tumors. Using the MCF-10A-series of cell lines that recapitulate the early stages of breast cancer formation and EMT, we found TWIST1 to be upregulated during EMT and downregulated early in carcinogenesis. The TWIST1 3'UTR contains putative regulatory elements, including miRNA target sites and two cytoplasmic polyadenylation elements (CPE). We found that miR-580, CPEB1, and CPEB2 act as negative regulators of TWIST1 expression in a sequence-specific and additive/cooperative manner.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Binding Sites
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Breast Neoplasms / metabolism*
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Breast Neoplasms / pathology
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Cell Line
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Cell Movement
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Disease Progression
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Epithelial-Mesenchymal Transition
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Female
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Gene Expression Regulation, Neoplastic*
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Genes, Reporter
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Humans
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Luciferases, Renilla / biosynthesis
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Luciferases, Renilla / genetics
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MicroRNAs / physiology*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Biosynthesis*
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RNA Interference
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RNA-Binding Proteins / metabolism
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RNA-Binding Proteins / physiology
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Transcription Factors / metabolism
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Transcription Factors / physiology
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Twist-Related Protein 1 / genetics
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Twist-Related Protein 1 / metabolism*
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Up-Regulation
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mRNA Cleavage and Polyadenylation Factors / metabolism
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mRNA Cleavage and Polyadenylation Factors / physiology
Substances
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3' Untranslated Regions
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CPEB1 protein, human
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CPEB2 protein, human
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MIRN580 microRNA, human
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MicroRNAs
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Nuclear Proteins
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RNA-Binding Proteins
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TWIST1 protein, human
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Transcription Factors
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Twist-Related Protein 1
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mRNA Cleavage and Polyadenylation Factors
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Luciferases, Renilla