Abstract
Embryonic stem cell (ESC)-specific microRNAs (miRNAs) play a critical role in the maintenance of pluripotency and self-renewal but the complete network between these miRNAs and their broad range of target genes still remains elusive. Here we demonstrate that miR-290 cluster, the most abundant miRNA family in ESCs, targets the NF-κB subunit p65 (also known as RelA) by repressing its translation. Forced expression of p65 causes loss of pluripotency, promotes differentiation of ESCs, and leads to an epithelial to mesenchymal transition. These data define p65 as a novel target gene of miR-290 cluster and provide new insight into the function of ESC-specific miRNAs.
Copyright © 2012 AlphaMed Press.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cell Differentiation / drug effects
-
Cell Differentiation / genetics
-
Cell Line
-
Epithelial-Mesenchymal Transition / drug effects
-
Epithelial-Mesenchymal Transition / genetics
-
HEK293 Cells
-
Humans
-
Mice
-
MicroRNAs / genetics
-
MicroRNAs / metabolism*
-
Multigene Family / genetics*
-
NF-kappa B / metabolism*
-
NIH 3T3 Cells
-
Phenotype
-
Pluripotent Stem Cells / cytology
-
Pluripotent Stem Cells / drug effects
-
Pluripotent Stem Cells / metabolism*
-
Protein Biosynthesis / drug effects
-
Protein Biosynthesis / genetics
-
Repressor Proteins / metabolism
-
Signal Transduction / drug effects
-
Signal Transduction / genetics*
-
Transcription Factor RelA / metabolism
-
Tretinoin / pharmacology
Substances
-
MicroRNAs
-
NF-kappa B
-
Repressor Proteins
-
Transcription Factor RelA
-
Tretinoin