Abstract
There was 100% solid tumor formation following inoculation of MCF-7 cells. However, MCF-7 tumor progression was significantly greater in the mice exposed to 17beta-estradiol (17beta-E2) compared to unexposed mice. WISP-2/CCN5 mRNA expression was correspondingly increased in 17beta-E2 exposed MCF-7 tumors compared to unexposed xenografts. Moreover, estrogen exposure followed by anti-estrogen tamoxifen treatment drastically inhibited the tumor growth and WISP-2 expression in nude mice. Therefore, the study suggests that higher WISP-2/CCN5 expression by estrogen may be associated with the estrogen-induced growth of MCF-7 tumors in vivo. Finally, overexpression of WISP-2/CCN5 may be considered as a prognostic marker of estrogen-sensitive tumor growth.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Breast Neoplasms / genetics*
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Breast Neoplasms / pathology*
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CCN Intercellular Signaling Proteins
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Disease Progression
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Estradiol / pharmacology*
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Female
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Gene Expression Regulation*
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Intercellular Signaling Peptides and Proteins / analysis
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Intercellular Signaling Peptides and Proteins / biosynthesis*
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Mice
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Mice, Nude
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Neoplasm Proteins / analysis
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Neoplasm Proteins / biosynthesis*
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Prognosis
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Repressor Proteins
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Signal Transduction
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Transcription Factors / analysis
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Transcription Factors / biosynthesis*
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Transplantation, Heterologous
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Tumor Cells, Cultured
Substances
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CCN Intercellular Signaling Proteins
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CCN5 protein, human
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Intercellular Signaling Peptides and Proteins
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Neoplasm Proteins
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Repressor Proteins
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Transcription Factors
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Estradiol