Abstract
Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double-stranded DNA. Structural studies have shown that LNA oligonucleotides induce A-type (RNA-like) duplex conformations. The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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DNA, Complementary / chemistry*
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DNA, Complementary / metabolism
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Endoribonucleases / chemistry
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Endoribonucleases / metabolism
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Hydrolysis
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Molecular Mimicry
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Nucleic Acid Conformation
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Nucleotides / chemistry*
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Nucleotides / metabolism
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Nucleotides / therapeutic use
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Oligonucleotides
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Oligonucleotides, Antisense / chemistry*
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Oligonucleotides, Antisense / metabolism
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Oligonucleotides, Antisense / therapeutic use
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RNA, Complementary / chemistry*
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RNA, Complementary / metabolism
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Ribose / analogs & derivatives*
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Ribose / chemistry*
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Ribose / metabolism
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Ribose / therapeutic use
Substances
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2'-O-4'-C-methylene-ribofuranosyl nucleotide
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DNA, Complementary
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Nucleotides
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Oligonucleotides
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Oligonucleotides, Antisense
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RNA, Complementary
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locked nucleic acid
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Ribose
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Endoribonucleases