Abstract
CD86 is an important costimulatory molecule for the priming and activation of naive and memory T cells, respectively. Here, we show that soluble CD86 is detected in human serum. Soluble CD86 is produced by resting monocytes and results from an alternatively spliced transcript (CD86deltaTM) characterized by deletion of the transmembrane domain. Recombinant CD86deltaTM binds to CD28 and CTLA-4 and induces the activation of T cells after stimulation with anti-CD3 mAb. CD86deltaTM also induces IFNgamma production by virus-specific CD8+ memory human T cells stimulated with the Flu M1 peptide. The concentrations of soluble CD86 found in human serum are sufficient to induce biological activity. Soluble CD86 molecule, therefore, appears to be a functional costimulatory molecule playing a potentially important role in immune surveillance.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, CD / biosynthesis
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Antigens, CD / blood
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Antigens, CD / genetics
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Antigens, CD / physiology*
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B7-2 Antigen
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COS Cells
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Epitopes, T-Lymphocyte / immunology
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Humans
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Immunologic Memory / genetics
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Interphase / immunology
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Jurkat Cells
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Lymphocyte Activation / genetics
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Lymphocyte Activation / immunology*
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Membrane Glycoproteins / biosynthesis
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Membrane Glycoproteins / blood
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Molecular Sequence Data
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Monocytes / immunology
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Monocytes / metabolism
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RNA Splicing / immunology
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RNA, Messenger / biosynthesis
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RNA, Messenger / metabolism
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Recombinant Proteins / biosynthesis
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Solubility
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T-Lymphocytes / immunology*
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Transcription, Genetic / immunology
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Transfection / immunology
Substances
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Antigens, CD
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B7-2 Antigen
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CD86 protein, human
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Epitopes, T-Lymphocyte
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Membrane Glycoproteins
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RNA, Messenger
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Recombinant Proteins