Abstract
We recently cloned a novel murine transcriptional repressor, the Krüppel-like zinc finger protein AP-2rep (HGMW-approved symbol KLF12), that binds to a regulatory element in the AP-2alpha gene promoter. In the present study, we characterize the human AP-2rep homolog and describe expression patterns in human urogenital and lymphoma cell lines. The predicted human protein of 402 amino acids exhibits 95.8% identity and 98.5% similarity to the murine AP-2rep peptide. The genomic locus of human AP-2rep consists of seven exons and was assigned to chromosome 13q22 by fluorescence in situ hybridization to metaphase chromosomes. Human AP-2rep repressed both reporter expression from a transiently transfected AP-2alpha promoter and the endogenous AP-2alpha gene and inversely was negatively regulated by AP-2alpha. The consensus motif CAGTGGG was identified by an in vitro binding site selection assay. In summary, our data further point to an important role of AP-2rep as a transcriptional silencer and reveal reciprocal regulation of AP-2alpha and AP-2rep.
Copyright 2000 Academic Press.
MeSH terms
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Amino Acid Sequence
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Animals
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Burkitt Lymphoma / metabolism
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Burkitt Lymphoma / pathology
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Cells, Cultured
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Chlorocebus aethiops
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Chromosomes, Human, Pair 13 / genetics
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Consensus Sequence
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DNA / metabolism*
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DNA, Complementary / genetics
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Gene Expression
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Gene Silencing
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Genes*
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HeLa Cells / metabolism
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Humans
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In Situ Hybridization, Fluorescence
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Jurkat Cells / metabolism
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Kidney / metabolism
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Kidney Neoplasms / metabolism
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Kidney Neoplasms / pathology
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Kruppel-Like Transcription Factors
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Male
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Mice
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Molecular Sequence Data
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Promoter Regions, Genetic
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / pathology
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Recombinant Fusion Proteins / physiology
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Sequence Alignment
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Sequence Homology, Amino Acid
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Species Specificity
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Teratocarcinoma / metabolism
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Teratocarcinoma / pathology
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transfection
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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DNA-Binding Proteins
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KLF12 protein, human
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Kruppel-Like Transcription Factors
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Neoplasm Proteins
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Recombinant Fusion Proteins
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Transcription Factors
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DNA