Laser Capture Microdissection: Beyond Functional Genomics to Proteomics
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Role of proteomics in surgical oncology
2023, Proteomics: A Promising Approach for Cancer ResearchA simple, cost-effective and flexible method for processing of snap-frozen tissue to prepare large amounts of intact RNA using laser microdissection
2012, BiochimieCitation Excerpt :The lack of RNA integrity in such studies has been criticized at best as unreliable and at worst as misleading [3–5]. The use of laser microdissection (LMD) [6] has transformed the acquisition of homogeneous cell populations for basic science [7–10], forensic science [1,2,11] and molecular medicine [7,12,13]. However, the quality and quantity of RNA recovered from samples obtained using this technique is quite low.
Nutriproteomics: A promising tool to link diet and diseases in nutritional research
2012, Biochimica et Biophysica Acta - Proteins and ProteomicsCitation Excerpt :Tissue culture samples treated with different compounds can be analyzed for their proteomes by the above described means. However, an analysis with variations in the biological sample such as different combinations of cell types can involve an initial separation of the different cells using cell specific surface markers and immunoaffinity techniques or laser driven microdissection approaches [26,139]. Apart from the analytical complexity, there are other limitations such as cost, resolution, reproducibility and throughput [5].
Protein analysis through Western blot of cells excised individually from human brain and muscle tissue
2012, Analytical BiochemistryProstate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction
2008, Human PathologyCitation Excerpt :An additional advantage is that LAM allows molecular analysis of cell populations in their native tissue environment and may be potentially applicable to biopsies obtained in preoperative diagnostic procedures. This new technique can thus overcome the problem of cellular heterogeneity that is a significant barrier to the molecular analysis of normal versus pathological tissues [10]. So far, few studies have reported on the protein content of microdissected tissue samples [11], mainly because of the limited sensitivity of protein immunoassays.
Proteomics: Methodologies and Applications in Oncology
2008, Seminars in Radiation OncologyCitation Excerpt :However, because tumors are characterized by both tumor cells and a range of host-derived cells, careful consideration must be made with respect to the starting material for comparison-based proteomic profiling. One approach is to use laser-capture microdissection to identify the areas within the tumor of interest and then to isolate the proteins specifically from this area before proteomic analysis with MS. This approach was first used to compare early- and late-stage disease to identify proteins associated with tumor progression using the SELDI machine.43 Although very few cells are required (as few as 25 will create a suitable MS peptide spectra), this is a rather tedious procedure and not easily amenable to high-throughput approaches.
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