Clinical–Alimentary TractPrognostic significance of calcium-binding protein S100A4 in colorectal cancer*,**
Section snippets
Materials and methods
For this study, all patients with primary CRC attending surgery between 1975 and 1995 in the Surgical Department of Hannover Medical School were reviewed (n = 1057). Exclusion criteria were no curative surgery (R1/R2 status: R1, 1% excluded; R2, 8% excluded), distant metastases at time of diagnosis/surgery (pM1, 6% excluded), age at surgery >75 years (15% excluded), postoperative lethality within 6 weeks (excluding patients who possibly died of surgery complications, 2% excluded), and no
Results
Of the 709 carcinomas evaluated, 114 (16%) showed high positivity, 223 (31%) showed low positivity, and 372 (53%) were negative for S100A4. Staining was present in the nucleus, as well as in the cytoplasm. Aside from S100A4-positive carcinoma cells, stromal cells, such as lymphocytes, endothelial cells, fibroblasts, and smooth muscle cells were also positively stained (Figure 1).
Compared with clinicopathologic parameters, significant association of high S100A4 positivity was observed with women
Discussion
To the best of our knowledge, this is the first study showing the prognostic relevance of S100A4 expression in CRC in a large series of cases. S100A4 was identified as a highly significant immunohistochemical marker, adding prognostic information to the clinical course of patients diagnosed with CRC. Interestingly, our results are in accordance with observations in other carcinomas in which S100A4 has been demonstrated to be of clinical relevance (lung cancer, esophageal cancer, or breast
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Address requests for reprints to: Reinhard von Wasielewski, M.D., Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, Hannover 30625, Germany. e-mail: [email protected]; fax: (49) 511 532 5799.
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Michael Mengel and Reinhard von Wasielewski declare a potential financial interest as inventors of a tissue microarray technique used in this study (patent pending).