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Caveolin-1 is regulated by c-myc and suppresses c-myc-induced apoptosis

Oncogene volume 19, pages 3256–3265 (2000)Cite this article

Abstract

Recent data indicating that overexpression of caveolin-1 as well as c-myc are relatively common features of advanced prostate cancer prompted us to test for potential cooperative interactions between caveolin-1 and c-myc that would be consistent with malignant progression. We used the well-characterized Rat1AmycERTM cells to show that the caveolin-1 gene is down-regulated at the level of transcription by c-myc. By maintaining relatively high levels of caveolin-1 with an adenoviral vector or in stably transfected clones we show that caveolin-1 can suppress c-myc-induced apoptosis. Further we established human prostate cancer cell lines with the mycERTM construct and show that clones with increased caveolin-1 are more resistant to myc-induced apoptosis and have increased capacity for growth in soft agar when c-myc is activated.

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Acknowledgements

This work was supported by grants from the National Institutes of Health (SPORE P50-CA58204 and RO-1, CA68814 and CA50588) and the Department of Defense (DAMD 17-98-1-8575). We thank Drs Craig Chinault, Joann Trial and Yanfuei Yuan for technical assistance.

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Authors and Affiliations

  1. Scott Department of Urology, Baylor College of Medicine, 6560 Fannin St., Suite 2100, Houston, 77030, Texas, TX, USA

    Terry L Timme, Alexei Goltsov, Salahaldin Tahir, Likun Li, Jianxiang Wang, Chengzhen Ren & Timothy C Thompson

  2. Departments of Cell Biology and Radiology, Baylor College of Medicine, One Baylor Plaza, Houston, 77030, Texas, TX, USA

    Timothy C Thompson

  3. Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, T2N 4N1, Alberta, Canada

    Randal N Johnston

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  1. Terry L Timme
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Timme, T., Goltsov, A., Tahir, S. et al. Caveolin-1 is regulated by c-myc and suppresses c-myc-induced apoptosis. Oncogene 19, 3256–3265 (2000). https://doi.org/10.1038/sj.onc.1203654

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