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Identification of a YAC from 16q24 carrying a senescence gene for breast cancer cells

Oncogene volume 19, pages 217–222 (2000)Cite this article

Abstract

We have identified a 360 kb YAC that carries a cell senescence gene, SEN16. In our earlier studies, we localized SEN16 within a genetic interval of 3–7 cM at 16q24.3. Six overlapping YACs spanning the chromosomal region of senescence activity, were assembled in a contig. Candidate YACs, identified by the markers located in the vicinity of SEN16, were retrofitted to introduce a neo selectable marker. Retrofitted YACs were first transferred into mouse A9 cells to generate A9/YAC hybrids. YAC DNA present in A9/YAC hybrids was further transferred by microcell fusion into immortal cell lines derived from human and rat mammary tumors. YAC d792t2 restored senescence in both human and rat mammary tumor cell lines, while an unrelated YAC from chromosome 6q had no senescence activity.

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Acknowledgements

This work was supported by grants from the American Cancer Society (ACS#RPG-97-138-01-MGO) and the American Federation for Aging Research (A96130) to G Pal Kaur. DE Reddy was supported by a predoctoral fellowship from US Army Breast Cancer Research Program (DAMD#17-96-1-6059). We thank Dr J Kim deRiel for suggestions and criticisms and Neena Rane for providing A9 hybrid for 966b10.

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Authors and Affiliations

  1. Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, 19140, Pennsylvania, PA, USA

    Deepthi E Reddy, Raghbir S Athwal & G Pal Kaur

  2. Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, 20892, Maryland, MD, USA

    Catherine L Keck & Nicholas Popescu

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  1. Deepthi E Reddy
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Reddy, D., Keck, C., Popescu, N. et al. Identification of a YAC from 16q24 carrying a senescence gene for breast cancer cells. Oncogene 19, 217–222 (2000). https://doi.org/10.1038/sj.onc.1203264

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