Abstract
Amplification of several chromosomal regions have been observed in human breast carcinomas. One such region, 8p11, is amplified in 10âââ15% of tumor samples. Although the FGFR1 gene is located close to this region, and is often included within the amplicon, the observation that tumors exhibiting 8p11 amplification do not always overexpress FGFR1 suggests that another gene located close to FGFR1 is involved in the tumorigenic process. We now report the precise location of four expressed sequence tags (ESTs) within this region and the cloning of a novel gene, designated TACC1 (transforming acidic coiled coil gene 1), which encodes an 8âkb transcript and which is expressed at high levels during early embryogenesis. Constitutive expression of this gene under the control of the cytomegalovirus (CMV) promoter in mouse fibroblasts, results in cellular transformation and anchorage independent growth, suggesting that inappropriate expression can impart a proliferative advantage. This observation raises the possibility that amplification of TACC1 could promote malignant growth, thereby making TACC1 an attractive candidate for the gene promoting tumorigenicity as a result of the 8p11 amplification in human breast cancers.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Adelaide J, Chaffenet M, Imbert A, Allione F, Geneix J, Popovici C, van Alewick D, Trapman J, Zeillinger R, Borresen-Dale A-L, Lidereau R, Birnbaum D and Pebusque M-J. . 1998 Genes Chrom. Cancer 22: 186â199.
Adnane J, Gaudray P, Dionne CA, Crumley G, Jaye M, Schlessinger J, Jeanteur P, Birnbaun D and Theillet C. . 1991 Oncogene 6: 659â663.
Courjal F, Cuny M, Simony-Lafontaine J, Louason G, Speiser P, Zeillinger R, Rodriguez C and Theillet C. . 1997 Cancer Res. 57: 4360â4367.
Dib A, Adelaide J, Chaffanet M, Imbert A, Le Paslier D, Jacquemier J, Gaudray P, Theillet C, Birnbaum D and Pebusque MJ. . 1995 Oncogene 10: 995â1001.
Escot C, Theillet C, Lidereau R, Spyratos F, Champeme MH, Gest J and Callahan R. . 1986 Proc. Natl. Acad. Sci. USA 83: 4834â4838.
Hawthorn LA, Roberts T, Verlind E, Kooy RF and Cowell JK. . 1995 Genomics 30: 425â430.
McLeskey SW, Ding IYF, Lippman ME and Kern FG. . 1994 Cancer Res. 54: 523â530.
Robbins J, Dilworth SM, Laskey RA and Dingwall C. . 1991 Cell 64: 615â623.
Sclessinger J and Ullrich A. . 1992 Neuron 9: 383â391.
Schuler GD, Boguski MS, Stewart EA, Stein LD, Gyapay G, Rice K, White RE, Rodriguez-Tome P, Agarwal A, Bajorek E, Bentolila S, Birren BB, Butler A, Castle AB, Chiannikulchai N, Chu A, Clee C, Cowles S, Day PJ and Dibling T, et al. 1996 Science 274: 540â546.
Schwartz MA. . 1997 J. Cell Biol. 139: 575â578.
Slamon D, Clark G, Wong S, Levin W, Ullrich A and McGuire W. . 1987 Science 235: 177â182.
Theillet C, Adnane J, Szepetowski P, Simon MP, Jeanteur P, Birnbaum D and Gaudray P. . 1990 Oncogene 5: 147â149.
Theillet C, Adelaide J, Louason G, Bonnet-Dorion F, Jacquemier J, Adnane J, Longy M, Katsaros D, Sismondi P, Gaudray P and Birnbaum D. . 1993 Genes Chrom. Cancer 7: 219â226.
Weskamp G, Kratzschmar J, Reid MS and Blobel CP. . 1996 J. Cell Biol. 132: 717â726.
Weyman CM and Stacey DW. . 1996 Adv. Oncobiol. 1: 159â177.
Acknowledgements
The first two authors contributed equally to this report. This work is in part supported by NIH grant GM49652 and AHA grant 96001110 to Alan Wolfman.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Still, I., Hamilton, M., Vince, P. et al. Cloning of TACC1, an embryonically expressed, potentially transforming coiled coil containing gene, from the 8p11 breast cancer amplicon. Oncogene 18, 4032â4038 (1999). https://doi.org/10.1038/sj.onc.1202801
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1202801
Keywords
This article is cited by
-
Genes and Pathways Involved in the Progression of Malignant Pleural Mesothelioma: A Meta-analysis of Genome-Wide Expression Studies
Biochemical Genetics (2023)
-
Repeats in Transforming Acidic Coiled-Coil (TACC) Genes
Biochemical Genetics (2013)
-
Analysis of Dictyostelium TACC reveals differential interactions with CP224 and unusual dynamics of Dictyostelium microtubules
Cellular and Molecular Life Sciences (2011)
-
The transforming acidic coiled coil (TACC1) protein modulates the transcriptional activity of the nuclear receptors TR and RAR
BMC Molecular Biology (2010)
-
High-resolution array CGH clarifies events occurring on 8p in carcinogenesis
BMC Cancer (2008)