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Structural study of fucoidan from Cladosiphon okamuranus tokida

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Abstract

A structural study was carried out on a fucoidan isolated from the brown seaweed Cladosiphon okamuranus. The polysaccharide contained fucose, glucuronic acid and sulfate in a molar ratio of about 6.1 : 1.0 : 2.9. The results of Smith degradation showed that this polysaccharide has a linear backbone of 1→3-linked α-fucopyranose with a half sulfate substitution at the 4-positions, and a portion of the fucose residues was O-acetylated. The data obtained from partial acid hydrolysis, a methylation analysis and NMR spectra indicated that the α-glucuronic acid residue is linked to the 2-positions of the fucose residues, which were not substituted by a sulfate group. These results indicated that the average structure of this fucoidan is as follows: -[(→3Fuc-4(±OSO3-)α1−)5→3[GlcAα1→2]Fucα1−]n−. (Half of each fucose residue was sulfated. One O-acetyl ester was present in every 6 fucose residues.)

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Authors and Affiliations

  1. Yakult Central Institute for Microbiological Research, Yaho 1796, Kunitachi-shi, Tokyo, 186-8650, Japan

    Masato Nagaoka, Hideyuki Shibata, Itsuko Kimura-Takagi, Shusuke Hashimoto, Kazumasa Kimura, Takashi Makino, Ritsuo Aiyama, Sadao Ueyama & Teruo Yokokura

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  1. Masato Nagaoka
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  2. Hideyuki Shibata
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  3. Itsuko Kimura-Takagi
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  4. Shusuke Hashimoto
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  5. Kazumasa Kimura
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  6. Takashi Makino
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  7. Ritsuo Aiyama
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  8. Sadao Ueyama
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  9. Teruo Yokokura
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Nagaoka, M., Shibata, H., Kimura-Takagi, I. et al. Structural study of fucoidan from Cladosiphon okamuranus tokida. Glycoconj J 16, 19–26 (1999). https://doi.org/10.1023/A:1006945618657

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