Biochemical and Biophysical Research Communications
Site-specific methylation of CpG nucleotides in the hTERT promoter region can control the expression of hTERT during malignant progression of colorectal carcinoma
Section snippets
Materials and methods
Patients, specimens, and cell lines. We analyzed 22 colorectal carcinoma tissues, five colon normal tissues, and the HCT 116 colon cancer cell line. These tissues of colorectal carcinoma and normal mucosa of 22 patients were obtained by surgical resection. All patients were diagnosed pathologically and no patient received radiotherapy or chemotherapy before the sampling. The samples were examined histologically for the presence of tumor cells. Clinical staging showed Duke’s A in one case,
hTERT expression in colorectal carcinomas is closely related with malignant progression
Recent investigations have demonstrated that hTERT activity is significantly increased in human malignant tumors, but is not expressed in normal somatic cells. Moreover, the quantification of hTERT expression holds promise for cancer prognosis as high telomerase activity has been correlated with a poor prognosis for a number of cancers [2], [3], [4], [17]. This suggests that hTERT activity and progression of malignant tumors are closely related.
Therefore, in our study, we investigated whether
Acknowledgment
This work was supported by Grants (R01-2004-000-10375-0) from the Korea Science and Engineering Foundation.
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