Elsevier

Analytical Biochemistry

Volume 344, Issue 1, 1 September 2005, Pages 141-143
Analytical Biochemistry

Notes & Tips
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

https://doi.org/10.1016/j.ab.2005.05.022Get rights and content

Section snippets

Acknowledgments

We thank Paul Kellam of the Department of Bioinformatics for advice and use of equipment. This work was funded by a grant from the British Lung Foundation. S.A. Bustin is grateful to Bowel and Cancer Research for financial support.

First page preview

First page preview
Click to open first page preview

References (9)

  • J. Huggett et al.

    Real-time RT–PCR normalisation: strategies and considerations

    Genes Immunol.

    (2005)
  • S.A. Bustin et al.

    Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction

    J. Biomol. Tech.

    (2004)
  • K. Dheda et al.

    Validation of housekeeping genes for normalizing RNA expression in real-time PCR

    BioTechniques

    (2004)
  • K. Dheda et al.

    Type2 cytokines in respiratory syncytial virus bronchiolitis

    Am. J. Respir. Crit. Care Med.

    (2004)
There are more references available in the full text version of this article.

Cited by (563)

View all citing articles on Scopus
View full text