Elsevier

Gene

Volume 85, Issue 2, 28 December 1989, Pages 445-451
Gene

Cloning of cDNA for a novel mouse membrane glycoprotein (gp42): shared identity to histocompatibility antigens, immunoglobulins and neural-cell adhesion molecules

https://doi.org/10.1016/0378-1119(89)90438-1Get rights and content

Abstract

A full-length clone encoding a murine membrane glycoprotein, gp42, was selected from a mouse fibroblast cDNA expression library by screening with a polyclonal antiserum. The deduced amino acid (aa) sequence indicates that gp42 is a transmembrane protein of 273 aa with a large N-terminal portion exposed outside the cell and a short cytoplasmic domain. Computer assisted analysis shows that gp42 is distinct from previously characterized proteins, but shares a number of structural features with the class II histocompatibility antigens. The sizes of the extracellular domains of gp42 and of class II histocompatibility antigens are similar, the position of four cysteines and the location of several aa residues are conserved. Some of these conserved residues are also present in immunoglobulins (Ig) and in the neural-cell adhesion molecule, thus indicating that gp42 is a new member of the Ig superfamily.

References (22)

  • L. Mengle-Gaw et al.

    Predicted protein sequence of the murine I-Eβs polypeptide chain from cDNA and genomic clones

  • Cited by (78)

    • The many faces of EMMPRIN-Roles in neuroinflammation

      2011, Biochimica et Biophysica Acta - Molecular Basis of Disease
      Citation Excerpt :

      During chicken CNS development, EMMPRIN is expressed in neuroblasts, but disappears from neurons upon differentiation, becoming restricted to capillary endothelial cells in the adult brain [19,20]. In contrast to chicken, in mouse and rat, EMMPRIN is differentially expressed in various sub-regions of the CNS suggesting that in mammals EMMPRIN has diverse functions other than contributions to the blood–brain barrier [10,21,22]. EMMPRIN expression has been reported in the olfactory system, hippocampal formation, septal area, amygdala, thalamic anterior nuclei, hypothalamus, mesencephalic tegmentum, entorhinal cortex, and cingulate gyrus EMMPRIN is also expressed in the retinal neuronal layers, the Vth layer of the cerebral neocortex, Purkinje cells of the cerebellum, several nuclei of the brain stem, and the gray matter of the spinal cord [22].

    • Disrupting the Amblyomma americanum (L.) CD147 receptor homolog prevents ticks from feeding to repletion and blocks spontaneous detachment of ticks from their host

      2010, Insect Biochemistry and Molecular Biology
      Citation Excerpt :

      The CD147 receptor, also called the extracellular matrix metalloprotease inducer (hence its other name, EMMPRIN) or the stromal cell derived receptor is a member of the immunoglobulin superfamily (Biswas and Nugent, 1987; Biswas et al., 1995; Ellis et al., 1989; Iacono et al., 2007; Nabeshima et al., 2006). The CD147 receptor, which was originally identified from human cancer cells (Biswas and Nugent, 1987), has now been characterized under a variety of names in several animals: OX-47 and CE9 in rats (Fossum et al., 1991; Nehme et al., 1995), gp42 and basigin in mice (Altruda et al., 1989; Miyauchi et al., 1991), HT7, neurothelin, and 5A11 antigen in chickens (Seulberger et al., 1990; Fadool and Linser, 1993). The CD147 receptor is mostly known for its role in stimulating matrix metalloproteases required for tumor invasion and metastasis (Iacono et al., 2007; Nabeshima et al., 2006).

    • CD147/basigin promotes progression of malignant melanoma and other cancers

      2010, Journal of Dermatological Science
      Citation Excerpt :

      Because of its ubiquitous distribution, it is thought to play fundamental roles in the regulation of various cellular activities. Molecules identical to Bsg were discovered and given different names, i.e. M6 [3] and EMMPRIN [4] in humans, HT7 [5], neurothelin [6], and 5A11 [7] in chickens, pg42 [8] in mice, and OX-47 [9] and CE9 [10] in rats. They turned out to be multifunctional and involved in various physiological processes such as fetal development, reproduction, T cell differentiation, and neural and retinal functions [11,12].

    View all citing articles on Scopus
    View full text