Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells: I. Microscopic observation of giant polynuclear cell formation

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Abstract

When an HVJ-ETC mixture is kept at 37 °C with shaking, polynuclear giant cells appear within 30 min. The process was observed under phase contrast and electron microscopes. By the addition of the virus at 4 °C the cells were strongly agglutinated and many virus particles were adsorbed onto the cell surface, especially onto microvilli. During incubation at 37 °C the virus particles disappeared from the cell surface and the large aggregates were dispersed into a number of smaller ones. At that time the degenerated cells which were contaminants from the original cell sample, and were included in the large aggregates, were rejected from the aggregated cells which are transformed into giant cells later. Then the opposed cells in the smaller aggregates fused together and after 30 min incubation typical giant cells appeared. The giant cells are due to fusion of the cells themselves. Two characteristic regions were observed in the reacting cells; one may be a part of the enfolded cell surface, and the other is a complexed projection on the cell surface at the contact point of opposed cells.

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