Purification of a hyaluronate-binding protein fraction that modifies cell social behavior

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Abstract

A hyaluronate-binding protein fraction (HABP) has been purified from the supernatant media of chick fibroblas cultures by ultrafiltration and Dowex-hyaluronate affinity chromatography. This protein fraction binds more avidly to Dowex-hyaluronate than to other Dowex-glycosaminoglycans. It can be inserted into the cell layer of urea-pretreated fibroblast monolayers where it specifically increases the amount of exogenous hyaluronate, but not sulfated glycosaminoglycans, attached to the cell glycocalyx. This interaction promotes a culture morphology and nuclear overlap ratio similar to virally-transformed cells. The principal hyaluronate-binding protein in the affinity purified fraction has a m.w. app. of 60,000–63,000 daltons.

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