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Upregulated PFTK1 promotes tumor cell proliferation, migration, and invasion in breast cancer

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Abstract

PFTK1 was a cell division cycle 2-related serine/threonine protein kinase, which was up-regulated in breast cancer tissues and breast cancer lines. And up-regulated PFTK1 was highly associated with grade, axillary lymph node status, and Ki-67. Moreover, Kaplan–Meier curve showed that up-regulated PFTK1 was related to the poor breast carcinoma patients’ overall survival. Here, we first discovered and confirmed that cyclin B was a new interacting protein of PFTK1, and the complex might increase the amount of DVL2, which triggers Wnt/β-catenin signaling pathway. Furthermore, knockdown of PFTK1 attenuated cell proliferation, anchorage-independent cell growth, and cell migration and invasion by inhibiting the transcriptional activation of β-catenin for cyclin D1, MMP9, and HEF1, whereas exogenous expression of PFTK1 might promote MDA-MB-231 cells proliferation, migration, and invasion via promoting PFTK1–DVL2–β-catenin axis. Our findings supported the notion that up-regulated PFTK1 might promote breast cancer progression and metastasis by activating Wnt signaling pathway through the PFTK1–DVL2–β-catenin axis.

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Acknowledgments

This study was funded by the National Natural Science Foundation of China Grants (Nos. 81302285, 81472185).

Conflict of interest

The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

      Xiaoling Gu & Guoxin Mao

    2. Department of Pathology, Nantong University Cancer Hospital, Nantong University, Nantong, 226001, Jiangsu, China

      Shuyun Yang

    3. Department of Pathogen Biology, Medical College, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu, China

      Xiaoling Gu, Yingying Wang, Jia Zhu, Wei Huang & Pan Xu

    4. Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

      Hua Wang, Qichao Ni & Chunhui Zhang

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    1. Xiaoling Gu
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    Correspondence to Guoxin Mao or Shuyun Yang.

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    Xiaoling Gu and Yingying Wang have contributed equally to this work.

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    Gu, X., Wang, Y., Wang, H. et al. Upregulated PFTK1 promotes tumor cell proliferation, migration, and invasion in breast cancer. Med Oncol 32, 195 (2015). https://doi.org/10.1007/s12032-015-0641-8

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    • DOI: https://doi.org/10.1007/s12032-015-0641-8

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