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miR-146b Regulates Cell Proliferation and Apoptosis in Gastric Cancer by Targeting PTP1B

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Abstract

Background/Aims

The purpose of this study is to explore the inhibition or activation effects of microRNA-146 B on the expression of PTP1B in gastric cancer cells.

Methods

The expressions of PTP1B and miR-146b in gastric cancer were detected by RT-qPCR. The effects of miR-146b on cell apoptosis and proliferation of gastric cancer were detected. The methods used in the detection process included Annexin V/PI dying method, colony formation assay, and MTT assay. The downstream target gene miR-146b was predicted and screened by bioinformatics and luciferase reporter assay. The mRNA and protein expressions of the target gene PTP1B miR-146b were determined using RT-qPCR and western blot. The expression of miR-146 B in mice was detected by the cells transfected with microRNA-146 B in vivo.

Results

Compared with normal tissues, PTP1B was higher and miR-146b was lower in cancer cells. Over-expression of miR-146b can inhibit cell viability and increase the apoptosis rate. According to the luciferase reporter assay, PTP1B was the downstream target gene of miR-146b. The re-introduction of PTP1B reversed the growth inhibition and apoptosis of gastric cancer cells induced by miR-146b. From the mouse xenograft model, the over-expression of miR-146b inhibited the tumor growth and reduced the expression level of PTP1B.

Conclusion

miR-146b directly inhibits the expression of PTP1B and suppressed the growth and development of gastric cancer.

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Funding

Project supported by Youth Talents Project of Science and Technology Research Program, Ministry of Education of Hubei Province, No. Q20142601.

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Author notes

  1. Jianguo Xu and Zilong Zhang have contributed equally to this work.

Authors and Affiliations

  1. Department of Oncology Surgery, People’s Hospital of Qinghai Province, Xining City, 810007, Qinghai Province, China

    Jianguo Xu & Zilong Zhang

  2. Department of Orthopedic, The 991 Hospital of PLA, Nanjing City, 441011, Jiangsu Province, China

    Qing Chen

  3. Department of Immunity, Medical College of Hubei University of Arts and Science, No. 296 Longzhong Road, Xiangyang City, 441053, Hubei Province, China

    Lin Yang & Jiao Yin

Authors
  1. Jianguo Xu
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  2. Zilong Zhang
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  3. Qing Chen
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  4. Lin Yang
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  5. Jiao Yin
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Correspondence to Jiao Yin.

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Xu, J., Zhang, Z., Chen, Q. et al. miR-146b Regulates Cell Proliferation and Apoptosis in Gastric Cancer by Targeting PTP1B. Dig Dis Sci 65, 457–463 (2020). https://doi.org/10.1007/s10620-019-05771-8

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  • DOI: https://doi.org/10.1007/s10620-019-05771-8

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