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Differential proteomic analysis of human colorectal carcinoma cell lines metastasis-associated proteins

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Abstract

Purpose

Metastasis is a common phenomenon and the major lethal cause of colorectal carcinoma (CRC). To better comprehend the mechanism underlying CRC metastasis and to search for potential markers for predicting CRC metastasis, two CRC cell lines with different metastatic potentials, SW480 and SW620, were investigated by phenotypic analyses and proteomics technologies.

Methods

The surgical orthotopic implantation (SOI) technique was originally used to develop a reproducible colorectal cancer model in nude mice with stable tumor growth and metastasizing course. Furthermore, differential proteome analysis of two CRC cell lines was conducted using two-dimensional (2-D) gel electrophoresis combined with matrix-assisted laser desorption/time of flight (MALDI-TOF) mass spectrometry.

Results

Among the differential spots, 12 protein spots (11 proteins) were further identified using in-gel tryptic digestion and peptide mass fingerprinting (PMF). Interestingly, most of these proteins we identified have been reported to be associated with distinct aspect of tumor metastasis to some extent. Our immunohistochemistry assays of colorectal cancer revealed that heat shock protein (HSP) 27 overexpression relates to metastatic behavior of CRC cell.

Conclusions

The protein profile between two colorectal cell lines with different potential metastasis displayed obvious differences. The results imply that various different proteins may lead to CRC metastasis together. HSP27 overexpression played an important role in metastasis and progression of CRC.

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Abbreviations

2-DE:

Two-dimensional electrphoresis

CPOX:

Coproporphyrinogen III oxidase

CRC:

Colorectal carcinoma

E-FABP:

Epidermal fatty acid binding protein

HMGB:

High-mobility group box

HSP:

Heat shock protein

MTAP:

Methylthioadenosine phosphorylase

PBP:

Posphatidylethanolamine binding protein

PGAM:

Phosphoglycerate mutase

MALDI-TOF:

Matrix-assisted laser desorption-ionization-time of flight

References

  • Aldrian S, Trautinger F, Frohlich I, Berger W, Micksche M, Kindas-Mugge I (2002) Overexpression of Hsp27 affects the metastatic phenotype of human melanoma cells in vitro. Cell Stress Chaperones 7(2):177–185

    Article  PubMed  CAS  Google Scholar 

  • Carlier MF, Wiesner S, Le Clainche C, Pantaloni D (2003) Actin-based motility as a self-organized system: mechanism and reconstitution in vitro. C R Biol 326(2):161–170

    Article  PubMed  CAS  Google Scholar 

  • Celis JE, Celis P, Ostergaard M, Basse B, Lauridsen JB, Ratz G, et al (1999) Proteomics and immunohistochemistry define some of the steps involved in the squamous differentiation of the bladder transitional epithelium: a novel strategy for identifying metaplastic lesions. Cancer Res 59(12):3003–3009

    PubMed  CAS  Google Scholar 

  • Chambers AF, Hill RP, Ling V (1981) Tumor heterogeneity and stability of the metastatic phenotype of mouse KHT sarcoma cells. Cancer Res 41(4):1368–1372

    PubMed  CAS  Google Scholar 

  • Choi YR, Kim H, Kang HJ, Kim NG, Kim JJ, Park KS, et al (2003) Overexpression of high mobility group box 1 in gastrointestinal stromal tumors with KIT mutation. Cancer Res 63(9):2188–2193

    PubMed  CAS  Google Scholar 

  • Dunnington DJ, Hughes CM, Monaghan P, Rudland PS (1983) Phenotypic instability of rat mammary tumor epithelial cells. J Natl Cancer Inst 71(6):1227–1240

    PubMed  CAS  Google Scholar 

  • Duranton B, Holl V, Schneider Y, Carnesecchi S, Gosse F, Raul F, et al (2003) Polyamine metabolism in primary human colon adenocarcinoma cells (SW480) and their lymph node metastatic derivatives (SW620). Amino Acids 24(1–2):63–72

    PubMed  CAS  Google Scholar 

  • Gagos S, Hopwood VL, Iliopoulos D, Kostakis A, Karayannakos P, Yatzides H, et al (1995) Chromosomal markers associated with metastasis in two colon cancer cell lines established from the same patient. Anticancer Res 15(2):369–378

    PubMed  CAS  Google Scholar 

  • Garrido C, Fromentin A, Bonnotte B, Favre N, Moutet M, Arrigo AP, et al (1998) Heat shock protein 27 enhances the tumorigenicity of immunogenic rat colon carcinoma cell clones. Cancer Res 58(23):5495–5499

    PubMed  CAS  Google Scholar 

  • Jiang P, Gan M, Huang H, Shen X, Wang S, Yao K (2005) Proteome analysis of antiproliferative mechanism of 12-O-tetradecanoylphorbol 13-acetate on cultured nasopharyngeal carcinoma CNE2 cells. J Proteome Res 4(2):599–605

    Article  PubMed  CAS  Google Scholar 

  • Laurich C, Wheeler MA, Iida J, Neudauer CL, McCarthy JB, Bullard KM (2004) Hyaluronan mediates adhesion of metastatic colon carcinoma cells. J Surg Res 122(1):70–74

    Article  PubMed  CAS  Google Scholar 

  • Leibovitz A, Stinson J, McCombs W, McCoy C, Mazur K, Mabry N (1976) Classification of human colorectal adenocarcinoma cell lines. Cancer Res 36(12):4562–4569

    PubMed  CAS  Google Scholar 

  • Lo Muzio L, Leonardi R, Mariggio MA, Mignogna MD, Rubini C, Vinella A, et al (2004) HSP 27 as possible prognostic factor in patients with oral squamous cell carcinoma. Histol Histopathol 19(1):119–128

    PubMed  CAS  Google Scholar 

  • Meyer G, Kim B, van Golen C, Feldman EL (2005) Cofilin activity during insulin-like growth factor I-stimulated neuroblastoma cell motility. Cell Mol Life Sci 62(4):461–470

    Article  PubMed  CAS  Google Scholar 

  • Mitsouras K, Wong B, Arayata C, Johnson RC, Carey M (2002) The DNA architectural protein HMGB1 displays two distinct modes of action that promote enhanceosome assembly. Mol Cell Biol 22(12):4390–4401

    Article  PubMed  CAS  Google Scholar 

  • Mouneimne G, Soon L, DesMarais V, Sidani M, Song X, Yip SC, et al (2004) Phospholipase C and cofilin are required for carcinoma cell directionality in response to EGF stimulation. J Cell Biol 166(5):697–708

    Article  PubMed  CAS  Google Scholar 

  • Perquin M, Oster T, Maul A, Froment N, Untereiner M, Bagrel D (2000) The glutathione-related detoxification pathway in the human breast: a highly coordinated system disrupted in the tumour tissues. Cancer Lett 158(1):7–16

    Article  PubMed  CAS  Google Scholar 

  • Ramagli LS (1999) Quantifying protein in 2-D PAGE solubilization buffers. Methods Mol Biol 112:99–103

    PubMed  CAS  Google Scholar 

  • Ryu JW, Kim HJ, Lee YS, Myong NH, Hwang CH, Lee GS, et al (2003) The proteomics approach to find biomarkers in gastric cancer. J Korean Med Sci 18(4):505–509

    PubMed  CAS  Google Scholar 

  • Stierum R, Gaspari M, Dommels Y, Ouatas T, Pluk H, Jespersen S, et al (2003) Proteome analysis reveals novel proteins associated with proliferation and differentiation of the colorectal cancer cell line Caco-2. Biochim Biophys Acta 1650(1–2):73–91

    PubMed  CAS  Google Scholar 

  • Stulik J, Hernychova L, Porkertova S, Knizek J, Macela A, Bures J, et al (2001) Proteome study of colorectal carcinogenesis. Electrophoresis 22(14):3019–3025

    Article  PubMed  CAS  Google Scholar 

  • Taguchi A, Blood DC, del Toro G, Canet A, Lee DC, Qu W, et al (2000) Blockade of RAGE-amphoterin signalling suppresses tumour growth and metastases. Nature 405(6784):354–360

    Article  PubMed  CAS  Google Scholar 

  • Thanner F, Sutterlin MW, Kapp M, Rieger L, Morr AK, Kristen P, et al (2005) Heat shock protein 27 is associated with decreased survival in node-negative breast cancer patients. Anticancer Res 25(3A):1649–1653

    PubMed  CAS  Google Scholar 

  • Tomonaga T, Matsushita K, Yamaguchi S, Oh-Ishi M, Kodera Y, Maeda T, et al (2004) Identification of altered protein expression and post-translational modifications in primary colorectal cancer by using agarose two-dimensional gel electrophoresis. Clin Cancer Res 10(6):2007–2014

    Article  PubMed  CAS  Google Scholar 

  • Varani J, Lovett EJ (1982) Phenotypic stability of murine tumor cells in vitro and in vivo. J Natl Cancer Inst 68(6):957–962

    PubMed  CAS  Google Scholar 

  • Wang H, Bloom O, Zhang M, Vishnubhakat JM, Ombrellino M, Che J, et al (1999) HMG-1 as a late mediator of endotoxin lethality in mice. Science 285(5425):248–251

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, et al (2005) Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol 168(3):441–452

    Article  PubMed  CAS  Google Scholar 

  • Yap CT, Simpson TI, Pratt T, Price DJ, Maciver SK (2005) The motility of glioblastoma tumour cells is modulated by intracellular cofilin expression in a concentration-dependent manner. Cell Motil Cytoskeleton 60(3):153–165

    Article  PubMed  CAS  Google Scholar 

  • Yu LR, Zeng R, Shao XX, Wang N, Xu YH, Xia QC (2000) Identification of differentially expressed proteins between human hepatoma and normal liver cell lines by two-dimensional electrophoresis and liquid chromatography-ion trap mass spectrometry. Electrophoresis 21(14):3058–3068

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by the Key Science and Technology Research Program of Guangdong Province (No.2003A308401) and the Natural Science Foundation of Guangdong Province (NO.5200512).

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Correspondence to Yan-qing Ding.

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Zhao, L., Liu, L., Wang, S. et al. Differential proteomic analysis of human colorectal carcinoma cell lines metastasis-associated proteins. J Cancer Res Clin Oncol 133, 771–782 (2007). https://doi.org/10.1007/s00432-007-0222-0

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  • DOI: https://doi.org/10.1007/s00432-007-0222-0

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