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Human colonic adenocarcinoma cells

I. Establishment and description of a new line

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Summary

A series of human colonic epithelial cell lines have been cultured from a single patient: LS-180 the original adenocarcinoma, LS-174T a trypsinized variant, and normal colonic tissue. The malignant cells, 20 to 40 µm in diameter and oval to polygonal, exhibited characteristics of normal colonic mucosal cells, namely, abundant microvilli prominent in secretory cells, and the presence of intracytoplasmic mucin vacuoles. The cultured adenocarcinoma cells, but not normal, demonstrated neoplastic properties by producing high levels of carcinoembryonic antigen (CEA) and by the ability to be propagated in hamster cheek pouches and in immunodeprived mice. The CEA production by the newly established line LS-180 released 900 times more CEA per cell into the culture medium and bore 30 times more cell-associated material than the established line, HT-29. These cell lines may permit detection of distinctive chemical, physiological, pharmacologic, and immunologic characteristics of neoplastic colonic cells.

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Authors and Affiliations

  1. Laboratory of Surgical Immunology of the Departments of Surgery and Physiology, Department of Pathology, Northwestern University Medical Center, Veterans Administration Lakeside Hospital, 60611, Chicago

    Baldwin H. Tom, Lynne P. Rutzky, Milda M. Jakstys, Ryoichi Oyasu, Celia I. Kaye & Barry D. Kahan

  2. Division of Genetics and Metabolism, Department of Pediatrics, Cook County Hospital, 60612, Chicago, Illinois

    Baldwin H. Tom, Lynne P. Rutzky, Milda M. Jakstys, Ryoichi Oyasu, Celia I. Kaye & Barry D. Kahan

Authors
  1. Baldwin H. Tom
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  2. Lynne P. Rutzky
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  3. Milda M. Jakstys
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  4. Ryoichi Oyasu
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  5. Celia I. Kaye
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  6. Barry D. Kahan
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Additional information

The use of the term “line” conforms with the recent definitions published by Fedoroff in theTissue Culture Association Manual, Vol. 1, No. 1, pp. 53–57, 1975.

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Tom, B.H., Rutzky, L.P., Jakstys, M.M. et al. Human colonic adenocarcinoma cells. In Vitro 12, 180–191 (1976). https://doi.org/10.1007/BF02796440

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  • DOI: https://doi.org/10.1007/BF02796440

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