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Antibody-Based Microarrays

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Book cover Microchip Methods in Diagnostics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 509))

Summary

Antibody-based microarrays are a new powerful proteomic technology that can be used to generate rapid and detailed expression profiles of defined sets of protein analytes in complex samples as well as high-resolution portraits of entire proteomes. Miniaturized micro- and nanoarrays can be printed with numerous antibodies carrying the desired specificities. Multiplexed and ultra-sensitive assays, specifically targeting several analytes in a single experiment, can be performed, while consuming only minute amounts of the sample. The array images generated can then be converted into protein expression profiles, or maps, revealing the detailed composition of the sample. This promising proteomic research tool will thus provide unique opportunities for e.g. disease proteomics, biomarker discovery, disease diagnostics, and patient stratification. This review describes the antibody-based microarray technology and applications thereof.

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Acknowledgments

This study was supported by grants from the Swedish National Science Council (VR-NT) and SSF, Strategic Center for Translational Cancer Research (CREATE Health) (www.createhealth.lth.se).

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Authors and Affiliations

  1. Department of Immunotechnology, Lund University, Lund, Sweden

    Christer Wingren & Carl A.K. Borrebaeck

  2. CREATE Health, Lund University, Lund, Sweden

    Christer Wingren & Carl A.K. Borrebaeck

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  1. Christer Wingren
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  2. Carl A.K. Borrebaeck
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Editors and Affiliations

  1. Helmholtz Centre for Infection Research, Braunschweig, Germany

    Ursula Bilitewski

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Wingren, C., Borrebaeck, C.A. (2009). Antibody-Based Microarrays. In: Bilitewski, U. (eds) Microchip Methods in Diagnostics. Methods in Molecular Biology™, vol 509. Humana Press. https://doi.org/10.1007/978-1-59745-372-1_5

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  • DOI: https://doi.org/10.1007/978-1-59745-372-1_5

  • Publisher Name: Humana Press

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