PT  - JOURNAL ARTICLE
AU  - GOUMARD, CLAIRE
AU  - DESBOIS-MOUTHON, CHRISTELE
AU  - WENDUM, DOMINIQUE
AU  - CALMEL, CLAIRE
AU  - MERABTENE, FATIHA
AU  - SCATTON, OLIVIER
AU  - PRAZ, FRANÇOISE
TI  - Low Levels of Microsatellite Instability at Simple Repeated Sequences Commonly Occur in Human Hepatocellular Carcinoma
DP  - 2017 Sep 01
TA  - Cancer Genomics - Proteomics
PG  - 329--339
VI  - 14
IP  - 5
4099  - http://cgp.iiarjournals.org/content/14/5/329.short
4100  - http://cgp.iiarjournals.org/content/14/5/329.full
SO  - Cancer Genomics Proteomics2017 Sep 01; 14
AB  - Background/Aim: The aim of this study was to assess the incidence of MSI in a large series of human hepatocellular carcinomas (HCC) with various etiologies. Materials and Methods: The MSI status was determined by polymerase chain reaction (PCR) using 5 mononucleotide and 13 CAn dinucleotide repeats. Results: None of the 122 HCC samples displayed an MSI-High phenotype, as defined by the presence of alterations at more than 30% of the microsatellite markers analyzed. Yet, limited microsatellite instability consisting in the insertion or deletion of a few repeat motifs was detected in 32 tumor samples (26.2%), regardless of the etiology of the underlying liver disease. MSI tended to be higher in patients with cirrhosis (p=0.051), possibly reflecting an impact of the inflammatory context in this process. Conclusion: Based on a large series of HCC with various etiologies, our study allowed us to definitely conclude that MSI is not a hallmark of HCC.