PT - JOURNAL ARTICLE AU - KALLIOPI I. PAPPA AU - VASILIKI LYGIROU AU - GEORGIA KONTOSTATHI AU - JEROME ZOIDAKIS AU - MANOUSOS MAKRIDAKIS AU - KONSTANTINOS VOUGAS AU - GEORGE DASKALAKIS AU - ALEXANDER POLYZOS AU - NICHOLAS P. ANAGNOU TI - Proteomic Analysis of Normal and Cancer Cervical Cell Lines Reveals Deregulation of Cytoskeleton-associated Proteins DP - 2017 Jul 01 TA - Cancer Genomics - Proteomics PG - 253--266 VI - 14 IP - 4 4099 - http://cgp.iiarjournals.org/content/14/4/253.short 4100 - http://cgp.iiarjournals.org/content/14/4/253.full SO - Cancer Genomics Proteomics2017 Jul 01; 14 AB - Background: Both HPV-positive and -negative cervical cancers are primarily associated with features of cell cycle and cytoskeletal disruption; however, the actual biological processes affected remain elusive. To this end, we systematically characterized the intracellular proteomic profiles of four distinct and informative cervical cell lines. Materials and Methods: Cell extracts from a normal cervical (HCK1T) and three cervical cancer cell lines, one HPV-negative (C33A), and two HPV-positive, SiHa (HPV16+) and HeLa (HPV18+), were analyzed by 2-dimensional electrophoresis and differentially expressed proteins were identified by MALDI-TOF mass spectrometry, while differential expression was confirmed by western blot analysis. Results: In total, 113 proteins were found differentially expressed between the normal and the cervical cancer lines. Bioinformatics analysis revealed the actin cytoskeleton signaling pathway to be significantly affected, while up-regulation of cofilin-1, an actin depolymerizing factor, was documented and further validated by western blotting. Furthermore, two-way comparisons among the four cell lines, revealed a set of 18 informative differentially expressed proteins. Conclusion: These novel identified proteins provide the impetus for further functional studies to dissect the mechanisms operating in the two distinct pathways of cervical carcinogenesis.