TY - JOUR T1 - Comparative Proteomics of Primary Breast Carcinomas and Lymph Node Metastases Outlining Markers of Tumor Invasion JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 89 LP - 101 VL - 12 IS - 2 AU - HELOISA HELENA MILIOLI AU - KARLA SANTOS SOUSA AU - RODRIGO KAVISKI AU - NAYARA CRISTINA DOS SANTOS OLIVEIRA AU - CÍCERO DE ANDRADE URBAN AU - RUBENS SILVEIRA DE LIMA AU - IGLENIR JOÃO CAVALLI AU - ENILZE MARIA DE SOUZA FONSECA RIBEIRO Y1 - 2015/03/01 UR - http://cgp.iiarjournals.org/content/12/2/89.abstract N2 - Background: Lymph node metastasis is an important clinicopathological parameter for breast cancer prognostication and treatment. Although the development of metastasis is common in axillary lymph nodes, the mechanisms underlying the locoregional spread are yet poorly understood. In the present study, we outline the involvement of proteins in tumor invasion by comparing the proteome profile of primary breast tumors (PBT) against that of lymph node metastasis (LNM). Patients and Methods: The comparative proteome analyses of seven paired samples were performed using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Results: Recurrent proteins were differentially expressed in PBT and LNM across patients. Higher levels of 1433G, 1433T, K2C8, PSME2, SNAA, TPM4, TRFE and VIME were observed in primary tumors compared to the metastatic site. On the other hand, higher levels of ALDH2 and GDIR2 were identified in metastasis related to tumors. These proteins provide a new insight on breast cancer research. Conclusion: Our achievements strengthened previous omics-based studies and also support the validation of potential markers of tumor invasion and metastasis. ER -