PT  - JOURNAL ARTICLE
AU  - KOU HAYAKAWA
AU  - TAKEAKI NAGAMINE
TI  - Fucoidan-dependent Increased Membrane Components in HepG2 Cells: Effect of Fucoidan Is Not Due to Gene Expression
DP  - 2014 Mar 01
TA  - Cancer Genomics - Proteomics
PG  - 93--113
VI  - 11
IP  - 2
4099  - http://cgp.iiarjournals.org/content/11/2/93.short
4100  - http://cgp.iiarjournals.org/content/11/2/93.full
SO  - Cancer Genomics Proteomics2014 Mar 01; 11
AB  - Background: The precise mechanism of the therapeutic effects of fucoidan (sulphated polyfucose) on cultured hepatocarcinoma HepG2 cells is as yet unclear. Materials and Methods: Protein components between fucoidan-treated and non-treated HepG2 cells were compared through a quantitative micro-sequencing method. Results: A dramatic and immediate increment of the membrane compartment and a decrement of RNA virus by fucoidan, as an effect of the Ishi-Mozuku (an edible brown seaweed Mozuku of Japan), are demonstrated. The ratio of membrane glycoproteins to total cellular proteins increases from 28.9% to 43.2% (1.5-fold), and the positive-sense single-stranded RNA viral proteins among the total cellular proteins decrease from 5.3% to 0.29% (18-fold), respectively, in response to 0.102 mg/ml fucoidan in HepG2 cells over three days’ period. Conclusion: Fucoidan seems to retard the growth of HepG2 cells through membrane glycoprotein metabolism. Therefore, fucoidan could be expected to have a therapeutic effect on hepatocellular carcinoma.