RT Journal Article
SR Electronic
T1 Genome-wide Transcriptional Sequencing Identifies Novel Mutations in Metabolic Genes in Human Hepatocellular Carcinoma
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 1
OP 12
VO 11
IS 1
A1 DAOUD M. MEERZAMAN
A1 CHUNHUA YAN
A1 QING-RONG CHEN
A1 MICHAEL N. EDMONSON
A1 CARL F. SCHAEFER
A1 ROBERT J. CLIFFORD
A1 BARBARA K. DUNN
A1 LI DONG
A1 RICHARD P. FINNEY
A1 CONSTANCE M. CULTRARO
A1 YING HU
A1 ZHIHUI YANG
A1 CU V. NGUYEN
A1 JENNY M. KELLEY
A1 SHUANG CAI
A1 HONGEN ZHANG
A1 JINGHUI ZHANG
A1 REBECCA WILSON
A1 LAUREN MESSMER
A1 YOUNG-HWA CHUNG
A1 JEONG A. KIM
A1 NEUNG HWA PARK
A1 MYUNG-SOO LYU
A1 IL HAN SONG
A1 GEORGE KOMATSOULIS
A1 KENNETH H. BUETOW
YR 2014
UL http://cgp.iiarjournals.org/content/11/1/1.abstract
AB We report on next-generation transcriptome sequencing results of three human hepatocellular carcinoma tumor/tumor-adjacent pairs. This analysis robustly examined ∼12,000 genes for both expression differences and molecular alterations. We observed 4,513 and 1,182 genes demonstrating 2-fold or greater increase or decrease in expression relative to their normal, respectively. Network analysis of expression data identified the Aurora B signaling, FOXM1 transcription factor network and Wnt signaling pathways pairs being altered in HCC. We validated as differential gene expression findings in a large data set containing of 434 liver normal/tumor sample pairs. In addition to known driver mutations in TP53 and CTNNB1, our mutation analysis identified non-synonymous mutations in genes implicated in metabolic diseases, i.e. diabetes and obesity: IRS1, HMGCS1, ATP8B1, PRMT6 and CLU, suggesting a common molecular etiology for HCC of alternative pathogenic origin.