RT Journal Article SR Electronic T1 The Translational Potential for Target Validation and Therapy Using Intracellular Antibodies in Oncology JF Cancer Genomics - Proteomics JO Cancer Genomics Proteomics FD International Institute of Anticancer Research SP 239 OP 250 VO 10 IS 6 A1 ULRICH H. WEIDLE A1 DANIELA MAISEL A1 ULRICH BRINKMANN A1 GEORG TIEFENTHALER YR 2013 UL http://cgp.iiarjournals.org/content/10/6/239.abstract AB Antibody-based molecules can be delivered into cells either by intracellular expression or through cellular uptake. We describe technologies for identification and expression of intracellular antibodies for target validation, intracellular immunization and tumor therapy, such as intracellular antibody capture technology, suicide or silencing technology, antigen-antibody interaction dependent apoptosis and their application for inhibition of oncogenic intracellular proteins and induction of apoptosis. These strategies have to be viewed in the context that inhibition of protein-protein interactions by small molecules is often limited due to their large interaction surface. We summarize antibodies with the ability to penetrate cells and strategies to induce uptake of antibodies after modification with protein transduction domains. Interference in oncogenic pathways is described for moieties based on antibody 3E10, which translocates into the nucleus after extracellular administration. Finally, we discuss examples of tumor immunotherapy and vaccination against intracellular antigens, and possible interactions mediating their mode of action.