TY - JOUR T1 - Ionizing Radiation Affects Epidermal Growth Factor Receptor Signalling and Metalloproteinase Secretion in Glioma Cells JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 33 LP - 38 VL - 8 IS - 1 AU - MARIA MARTINOU AU - EFSTATHIA GIANNOPOULOU AU - GEORGIA MALATARA AU - ANDREAS A. ARGYRIOU AU - HARALABOS P. KALOFONOS AU - DIMITRIOS KARDAMAKIS Y1 - 2011/01/01 UR - http://cgp.iiarjournals.org/content/8/1/33.abstract N2 - Background: The effect of different doses of X(-)rays on apoptosis, proliferation, epidermal growth factor receptor (EGFR) and matrix metalloproteinase (MMP-2) expression was investigated in a human glioblastoma cell line. Materials and Methods: The cell line LN18 was irradiated at room temperature with doses ranging from 0.5 to 15 Gy using 6 MV X(-)rays. Apoptosis was assessed using the annexin V binding assay, proliferation by the methyl tetrazolium (MTT) assay and MMP-2 secretion with zymography. The levels of phosphorylated (pEGFR) were estimated using a commercially available ELISA kit. Results: Cell proliferation decreased in a dose-dependent manner, while apoptosis was increased after radiation. Doses below 2 Gy did not affect proliferation or apoptosis. MMP-2 levels were increased 48 h after radiation in a dose-dependent manner. In contrast, EGFR signaling was significantly activated 15 min after radiation in a dose-dependent manner. Conclusion: Ionizing radiation activates EGFR signalling and enhances MMP-2 secretion, suggesting that the molecular pathways involved may contribute to the invasiveness and malignant behaviour of glioma cells and help to explain the response of gliomas to ionizing radiation. ER -