RT Journal Article
SR Electronic
T1 Genomic Analysis of the 55 kDa Subunit of DNA Polymerase ϵ in Human Intracranial Neoplasms
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 143
OP 146
VO 7
IS 3
A1 ZHOU, QI
A1 LEHTONEN, TUOMO
A1 LEHTONEN, JAANA
A1 LAAKSO, SUSANA
A1 SZYMAS, JANUSZ
A1 POSPIECH, HELMUT
A1 HUOPONEN, KIRSI
A1 SYVÄOJA, JUHANI
A1 COLLAN, YRJÖ
YR 2010
UL http://cgp.iiarjournals.org/content/7/3/143.abstract
AB Background: Defects of some DNA polymerases have shown cancer associations, but there are only limited data on DNA polymerase (Pol) ϵ. Materials and Methods: We examined 26 human brain neoplasm DNA samples and 8 control blood samples (from Poland) for possible mutations in the entire coding region of the 55 kDa small subunit of human DNA Pol ϵ gene using polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) analysis, and sequence analysis of DNA. Results: One single base intronic transition in intron 14 was found. The AATT deletion previously found in some breast and colorectal tumors was not found in samples from brain neoplasms or controls, but it was found in 1/100 normal blood samples from South-West Finland. Conclusion: We found no evidence that potential mutations in the 55 kDa subunit of DNA Pol ϵ are a contributing factor in the development of the tested cases of human intracranial tumors.