RT Journal Article SR Electronic T1 Spontaneous In Vitro Transformation of Primary Human Osteoblast-like Cells JF Cancer Genomics - Proteomics JO Cancer Genomics Proteomics FD International Institute of Anticancer Research SP 61 OP 66 VO 7 IS 2 A1 URSULA MAGDOLEN A1 MANFRED SCHMITT A1 BARBARA HILDEBRANDT A1 PETER DIEHL A1 JOHANNES SCHAUWECKER A1 BELMA SALDAMLI A1 RAINER BURGKART A1 JUTTA TÜBEL A1 RAINER GRADINGER A1 BRIGITTE ROYER-POKORA YR 2010 UL http://cgp.iiarjournals.org/content/7/2/61.abstract AB Background: Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery. Materials and Methods: The tumor cell properties studied included morphology, proliferation characteristics in normal and low-serum media, and anchorage-independent growth in soft agarose. Results and Conclusion: Karyotyping of the cells showed numerous rearrangements and abnormalities. These results pointed to the tumorigenic potential of the cells and demonstrate the importance of biosafety in tissue engineering and therapeutic cell applications when prolonged culture conditions are required.