RT Journal Article
SR Electronic
T1 Screening for Epigenetically Masked Genes in Colorectal Cancer Using 5-Aza-2’-deoxycytidine, Microarray and Gene Expression Profile
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 67
OP 75
VO 9
IS 2
A1 Khamas, Ahmed
A1 Ishikawa, Toshiaki
A1 Shimokawa, Kazuro
A1 Mogushi, Kaoru
A1 Iida, Satoru
A1 Ishiguro, Megumi
A1 Mizushima, Hiroshi
A1 Tanaka, Hiroshi
A1 Uetake, Hiroyuki
A1 Sugihara, Kenichi
YR 2012
UL http://cgp.iiarjournals.org/content/9/2/67.abstract
AB Aim: Unearthing of silenced genes in colorectal cancer (CRC). Materials and Methods: Oligonucleotide microarray was used in order to find changes in gene expression in five CRC cell lines before and after 5-aza-2’-Deoxycitidine treatment. Up-regulated genes were integrated with expression profile of matched colorectal tissue samples. Methylation-specific polymerase chain reaction and Real-time quantitative reverse transcription polymerase chain reaction were used to further analyze candidates using 15 CRC cell lines and 23 paired samples. Results: After applying study selection criteria for 68 genes obtained from integrated arrays, we identified 16 genes; apoptosis-stimulating of p53 protein 1(ASPP1) and Scavenger receptor class A, member 5 (SCARA5) were selected for further analysis. Methylation was only identified for SCARA5 in 20% of the cell lines and in 17% of tumor the samples. Down expression of SCARA5 was observed in CRC cell lines and in tumor samples compared to normal (p<0.001 and p=0.001, respectively). Conclusion: Genome-wide screening identifies genes potentially affected by methylation in CRC. SCARA5 may have a role in tumorigenesis in CRC.