PT  - JOURNAL ARTICLE
AU  - Khamas, Ahmed
AU  - Ishikawa, Toshiaki
AU  - Shimokawa, Kazuro
AU  - Mogushi, Kaoru
AU  - Iida, Satoru
AU  - Ishiguro, Megumi
AU  - Mizushima, Hiroshi
AU  - Tanaka, Hiroshi
AU  - Uetake, Hiroyuki
AU  - Sugihara, Kenichi
TI  - Screening for Epigenetically Masked Genes in Colorectal Cancer Using 5-Aza-2’-deoxycytidine, Microarray and Gene Expression Profile
DP  - 2012 Mar 01
TA  - Cancer Genomics - Proteomics
PG  - 67--75
VI  - 9
IP  - 2
4099  - http://cgp.iiarjournals.org/content/9/2/67.short
4100  - http://cgp.iiarjournals.org/content/9/2/67.full
SO  - Cancer Genomics Proteomics2012 Mar 01; 9
AB  - Aim: Unearthing of silenced genes in colorectal cancer (CRC). Materials and Methods: Oligonucleotide microarray was used in order to find changes in gene expression in five CRC cell lines before and after 5-aza-2’-Deoxycitidine treatment. Up-regulated genes were integrated with expression profile of matched colorectal tissue samples. Methylation-specific polymerase chain reaction and Real-time quantitative reverse transcription polymerase chain reaction were used to further analyze candidates using 15 CRC cell lines and 23 paired samples. Results: After applying study selection criteria for 68 genes obtained from integrated arrays, we identified 16 genes; apoptosis-stimulating of p53 protein 1(ASPP1) and Scavenger receptor class A, member 5 (SCARA5) were selected for further analysis. Methylation was only identified for SCARA5 in 20% of the cell lines and in 17% of tumor the samples. Down expression of SCARA5 was observed in CRC cell lines and in tumor samples compared to normal (p<0.001 and p=0.001, respectively). Conclusion: Genome-wide screening identifies genes potentially affected by methylation in CRC. SCARA5 may have a role in tumorigenesis in CRC.