PT  - JOURNAL ARTICLE
AU  - FRIEDHELM R. SCHUSTER
AU  - RAYMUND BUHMANN
AU  - SUSANNE REUTHER
AU  - BERND HUBNER
AU  - CHRISTINE GRABRUCKER
AU  - ANJA LIEPERT
AU  - ROLAND REIBKE
AU  - PETER LICHTNER
AU  - TING YANG
AU  - TANJA KROELL
AU  - HANS-JOCHEM KOLB
AU  - ARNDT BORKHARDT
AU  - HELGA SCHMETZER
TI  - Improved Effector Function of Leukemia-specific T-lymphocyte Clones Trained with AML-derived Dendritic Cells
DP  - 2008 Sep 01
TA  - Cancer Genomics - Proteomics
PG  - 275--286
VI  - 5
IP  - 5
4099  - http://cgp.iiarjournals.org/content/5/5/275.short
4100  - http://cgp.iiarjournals.org/content/5/5/275.full
SO  - Cancer Genomics Proteomics2008 Sep 01; 5
AB  - Recently it was shown that myeloid leukemic cells can be induced to differentiate into leukemia-derived dendritic cells (DCleu), regaining the stimulatory capacity of professional DCs while presenting the leukemic antigen repertoire. But so far, the induced antileukemic T-cell responses have varied in specificity and efficacy, or have even mediated opposite effects. In an attempt to further characterize the DC/DCleu induced T-cell response pattern, immunoscope spectratyping, a novel and powerful tool to detect T-cell receptor (TCR) rearrangements was used in combination with functional flow cytometry and non-radioactive fluorolysis assays. Human leucocyte antigen (HLA) matched donor T-cells were repeatedly stimulated, either with leukemic blasts (French-American-British, FAB M4eo) or the corresponding blast-derived DCs. Functional comparison revealed no significant difference in their T-cell stimulatory capacity, while the DC/DCleu fraction favored T-cells with a higher lytic activity, comprising a higher proportion of T-memory CD45R0+ cells. Stimulation with blasts and DC/DCleu induced a similar TCR restriction pattern, while stimulation with DC/DCleu favored the CD4 T-cell subset and seemed to cause a higher grade of restriction. In conclusion, a combined strategy using spectratyping with functional tests might not only provide useful information about the specificity and efficacy of the induced T-cell response, but also pave the way to gain effective T-cell clones for therapeutic use.