RT Journal Article
SR Electronic
T1 Proteomic Analysis of Liver from Transgenic Mice Overexpressing Small Heterodimer Partner
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 119
OP 126
VO 3
IS 2
A1 KOUYIANOU, KALLIA
A1 GARBIS, SPYROS
A1 BOULIAS, KONSTANTINOS
A1 DIMITRAKI, PLOUMISTI
A1 FOUNTOULAKIS, MICHAEL
A1 TSIOTIS, GEORGIOS
YR 2006
UL http://cgp.iiarjournals.org/content/3/2/119.abstract
AB The small heterodimer partner (SHP) is a key regulator of genes involved in cholesterol-bile acid homeostasis and functions as a specific transcription repressor. Differential protein expression in the liver of transgenic mice expressing the human SHP gene was compared with wild-type animals. Liver protein extracts were analyzed by two-dimensional electrophoresis and the proteins were identified by MALDI-TOF-MS. Approximately 30 proteins were differentially-expressed in the livers of transgenic mice, compared to the control mice. Major effects were evident in lipid accumulation, including a fatty acid-binding protein. Overexpression of SHP also triggered alterations in key enzymes involved in the metabolism of amino acids, nucleic acids and urea and was associated with changes in cellular proteins involved in calcium homeostasis, detoxification and protein folding and repair.