PT  - JOURNAL ARTICLE
AU  - KOUYIANOU, KALLIA
AU  - GARBIS, SPYROS
AU  - BOULIAS, KONSTANTINOS
AU  - DIMITRAKI, PLOUMISTI
AU  - FOUNTOULAKIS, MICHAEL
AU  - TSIOTIS, GEORGIOS
TI  - Proteomic Analysis of Liver from Transgenic Mice Overexpressing Small Heterodimer Partner
DP  - 2006 Mar 01
TA  - Cancer Genomics - Proteomics
PG  - 119--126
VI  - 3
IP  - 2
4099  - http://cgp.iiarjournals.org/content/3/2/119.short
4100  - http://cgp.iiarjournals.org/content/3/2/119.full
SO  - Cancer Genomics Proteomics2006 Mar 01; 3
AB  - The small heterodimer partner (SHP) is a key regulator of genes involved in cholesterol-bile acid homeostasis and functions as a specific transcription repressor. Differential protein expression in the liver of transgenic mice expressing the human SHP gene was compared with wild-type animals. Liver protein extracts were analyzed by two-dimensional electrophoresis and the proteins were identified by MALDI-TOF-MS. Approximately 30 proteins were differentially-expressed in the livers of transgenic mice, compared to the control mice. Major effects were evident in lipid accumulation, including a fatty acid-binding protein. Overexpression of SHP also triggered alterations in key enzymes involved in the metabolism of amino acids, nucleic acids and urea and was associated with changes in cellular proteins involved in calcium homeostasis, detoxification and protein folding and repair.