RT Journal Article SR Electronic T1 Specific Expression of Potential Tumour Marker Proteins, Similar to No On or Off Transient A and HIRA-interacting Protein 5, in Mouse N1E-115 Neuroblastoma Cell Line JF Cancer Genomics - Proteomics JO Cancer Genomics Proteomics FD International Institute of Anticancer Research SP 209 OP 218 VO 2 IS 4 A1 JI-EUN OH A1 EPHREM ENGIDAWORK A1 JOO-HO SHIN A1 GERT LUBEC YR 2005 UL http://cgp.iiarjournals.org/content/2/4/209.abstract AB Background: Improved cancer detection involving suitable biomarkers with easy applicability is a challenge in our fight against cancer. Biomarkers that provide significant insight into the behaviour of neuroblastoma would greatly aid in identifying patients at risk of disease progression, those whose disease has progressed sub-clinically and those who would benefit from currently available systemic therapies. Materials and Methods: Matrix-assisted laser desorption and ionization-time-of-flight (MALDI-TOF-TOF) is an evolving proteomic technology for improving biomarker discovery, that allows for rapid and sensitive analysis of complex protein mixtures generated from body fluids, cells and/or tissues. MALDI-based profiling identifies unique, differentially-expressed proteins relating to specific cancer-related disease states. A proteomic map of the murine neuroblastoma N1E-115 cell line was generated and MALDI-TOF-TOF utilized in a search for tumour marker candidates. Results: The analytical tool identified and characterized similar to no on or off transient A [fragment] and HIRA-interacting protein 5 proteins that have never been described in any normal or tumour cell lines. Conclusion: These findings suggest that the proteins may serve as candidate markers for screening, staging and drug selection for the management of neuroblastoma, owing to their tumour-specific expression.