<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">RACHID, OUSAMA</style></author><author><style face="normal" font="default" size="100%">ALKHALAF, MOUSSA</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Resveratrol Regulation of PI3K-AKT Signaling Pathway Genes in MDA-MB-231 Breast Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Cancer Genomics - Proteomics</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006-11-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">383-388</style></pages><volume><style face="normal" font="default" size="100%">3</style></volume><issue><style face="normal" font="default" size="100%">6</style></issue><abstract><style  face="normal" font="default" size="100%">Background: Resveratrol (RSVL), a natural compound found in grapes and other food products, has been described to exert cancer chemopreventive activities. However, the cellular and molecular basis of its anticancer activity is largely undefined. The aim of the present study was to identify RSVL target genes in the MDA-MB-231 breast cancer cell line using cDNA arrays representing genes of the PI3K/AKT signaling pathway. Materials and Methods: Total RNA from control and RSVL-treated cells was used to synthesize biotinylated cDNA probes. cDNA arrays were hybridized with the probes and signals were detected with a chemiluminescent method. Western blotting analysis was used to validate the arrays' gene expression. Results: At the cDNA level, 13 genes were altered (at least 2-fold difference) by RSVL treatment. At the protein level, both c-fos and P70S6K were also regulated. Conclusion: Using gene arrays it was shown for the first time that c-fos and p70S6 kinase were regulated by RSVL.</style></abstract></record></records></xml>