PT  - JOURNAL ARTICLE
AU  - HARDOUIN, JULIE
AU  - CANELLE, LUDOVIC
AU  - VLIEGHE, CÉLINE
AU  - LASSERRE, JEAN-PAUL
AU  - CARON, MICHEL
AU  - JOUBERT-CARON, RAYMONDE
TI  - Proteomic Analysis of the MCF7 Breast Cancer Cell Line
DP  - 2006 Nov 01
TA  - Cancer Genomics - Proteomics
PG  - 355--368
VI  - 3
IP  - 6
4099  - http://cgp.iiarjournals.org/content/3/6/355.short
4100  - http://cgp.iiarjournals.org/content/3/6/355.full
SO  - Cancer Genomics Proteomics2006 Nov 01; 3
AB  - Background: The MCF7 breast cancer cell line is a cellular model for breast cancer studies and marker discovery. Therefore, a better knowledge of its proteome is a prerequisite for a more efficient use of this model. Materials and Methods: Proteins expressed during the exponential growth phase of MCF7 cells were analyzed and mapped using two-dimensional gel electrophoresis and mass spectrometry. Results: From the spots excised from preparative gels of whole-cell extracts, a subset of 368 different polypeptides, corresponding to 249 different proteins, was identified. These polypeptides were positioned on a silver-stained gel to construct a reference map. Conclusion: The data allowed the construction of the most extensive reference map for MCF7 published to date, with 189 novel proteins, which had not been previously listed on maps, and are now accessible on World 2D-PAGE database, providing a basis for further studies on MCF7.