RT Journal Article
SR Electronic
T1 Cadmium Induces Fas Down-Regulation in a Human Immature T-cell Line
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 77
OP 86
VO 1
IS 1
A1 GEORGE TH. TSANGARIS
A1 ATHANASIOS BOTSONIS
A1 IOANNIS POLITIS
A1 FOTINI TZORTZATOU-STATHOPOULOU
YR 2004
UL http://cgp.iiarjournals.org/content/1/1/77.abstract
AB Cadmium (Cd2+) is an ubiquitous toxic metal with apoptotic and genotoxic effects, which has been involved in a variety of pathological conditions inducing disturbance of the immune system. In the present study we treated the Fas-expressed human immature T-cell line CCRF-CEM with 10μM Cd2+ for 6h or 24h. We found that pretreatment of the cells with Cd2+ for 24h inhibited apoptosis induced by Fas-ligation with the CH-11 antibody, in contrast to pretreatment for 6h. Immunocytochemical and multiplex RT-PCR analyses indicated that Cd2+ treatment for 24h inhibited Fas expression at the transcriptional level. To investigate that effect of Cd2+, cDNA microarray analysis was applied, which indicated that the rapid induction of NF-kB, ERK5 and JAK3 genes by Cd2+ was the initial step resulting in Fas down-regulation. The Fas down-regulation induced by Cd2+ seems to be responsible for the carcinogenic and the immunomodulatory effects of that metal.