RT Journal Article
SR Electronic
T1 Over-methylation of Histone H3 Lysines Is a Common Molecular Change Among the Three Major Types of Soft-tissue Sarcoma in Patient-derived Xenograft (PDX) Mouse Models
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 715
OP 721
DO 10.21873/cgp.20292
VO 18
IS 6
A1 YUSUKE AOKI
A1 JUN YAMAMOTO
A1 YASUNORI TOME
A1 KAZUYUKI HAMADA
A1 NORIYUKI MASAKI
A1 SACHIKO INUBUSHI
A1 YOSHIHIKO TASHIRO
A1 MICHAEL BOUVET
A1 ITARU ENDO
A1 KOTARO NISHIDA
A1 ROBERT M. HOFFMAN
YR 2021
UL http://cgp.iiarjournals.org/content/18/6/715.abstract
AB Background/Aim: Sarcomas are considered a heterogeneous disease with incomplete understanding of its molecular basis. In the present study, to further understand general molecular changes in sarcoma, patient-derived xenograft (PDX) mouse models of the three most common soft-tissue sarcomas: myxofibrosarcoma, undifferentiated pleomorphic sarcoma (UPS) and liposarcoma were established and the methylation status of histone H3 lysine marks was studied. Materials and Methods: Immunoblotting and immunohistochemical staining were used to quantify the extent of methylation of histone H3K4me3 and histone H3K9me3. Results: In all 3 sarcoma types in PDX models, histone H3K4me3 and H3K9me3 were found highly over-methylated compared to normal muscle tissue. Conclusion: Histone H3 lysine over-methylation may be a general basis of malignancy of the major sarcoma types.