PT  - JOURNAL ARTICLE
AU  - YUSUKE AOKI
AU  - JUN YAMAMOTO
AU  - YASUNORI TOME
AU  - KAZUYUKI HAMADA
AU  - NORIYUKI MASAKI
AU  - SACHIKO INUBUSHI
AU  - YOSHIHIKO TASHIRO
AU  - MICHAEL BOUVET
AU  - ITARU ENDO
AU  - KOTARO NISHIDA
AU  - ROBERT M. HOFFMAN
TI  - Over-methylation of Histone H3 Lysines Is a Common Molecular Change Among the Three Major Types of Soft-tissue Sarcoma in Patient-derived Xenograft (PDX) Mouse Models
AID  - 10.21873/cgp.20292
DP  - 2021 Nov 01
TA  - Cancer Genomics - Proteomics
PG  - 715--721
VI  - 18
IP  - 6
4099  - http://cgp.iiarjournals.org/content/18/6/715.short
4100  - http://cgp.iiarjournals.org/content/18/6/715.full
SO  - Cancer Genomics Proteomics2021 Nov 01; 18
AB  - Background/Aim: Sarcomas are considered a heterogeneous disease with incomplete understanding of its molecular basis. In the present study, to further understand general molecular changes in sarcoma, patient-derived xenograft (PDX) mouse models of the three most common soft-tissue sarcomas: myxofibrosarcoma, undifferentiated pleomorphic sarcoma (UPS) and liposarcoma were established and the methylation status of histone H3 lysine marks was studied. Materials and Methods: Immunoblotting and immunohistochemical staining were used to quantify the extent of methylation of histone H3K4me3 and histone H3K9me3. Results: In all 3 sarcoma types in PDX models, histone H3K4me3 and H3K9me3 were found highly over-methylated compared to normal muscle tissue. Conclusion: Histone H3 lysine over-methylation may be a general basis of malignancy of the major sarcoma types.