TY - JOUR T1 - <em>OIP5-AS1</em> Promotes Proliferation of Non-small-cell Lung Cancer and Head and Neck Squamous Cell Carcinoma Cells JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 543 LP - 548 DO - 10.21873/cgp.20279 VL - 18 IS - 4 AU - YOJIRO KOTAKE AU - NATSUMI MATSUNAGA AU - TAKAHIRO WAKASAKI AU - RYOTARO OKADA Y1 - 2021/07/01 UR - http://cgp.iiarjournals.org/content/18/4/543.abstract N2 - Background/Aim: The long noncoding RNA OIP5 antisense RNA 1 (OIP5-AS1) is overexpressed in various cancer types, such as lung cancer, hepatoblastoma and cervical cancer, and functions to accelerate cell proliferation, invasion and migration. Here, we investigated the roIe of OIP5-AS1 in cell-cycle progression of H1299 and A549 non-small cell lung cancer cells, and FaDu and CAL27 head and neck squamous cell carcinoma cells. Materials and Methods: The cells were transfected with small interfering RNA and subjected to cell-cycle analysis and reverse-transcription quantitative polymerase chain reaction (RT-qPCR). Results: Silencing of OIP5-AS1 suppressed the proliferation of H1299, A549, FaDu and CAL27 cells. RT-qPCR and cell-cycle analysis revealed that silencing OIP5-AS1 increased the expression of CDK inhibitors, such as p15, p16, p18 and p19, resulting in G1-phase arrest. Conclusion: OIP5-AS1 regulates G1-phase progression by repressing CDK inhibitors and, thus, promotes the proliferation of H1299, A549, FaDu and CAL27 cells. ER -