TY - JOUR T1 - Profiling of Serum Extracellular Vesicles Reveals <em>miRNA-4525</em> as a Potential Biomarker for Advanced Renal Cell Carcinoma JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 253 LP - 259 DO - 10.21873/cgp.20256 VL - 18 IS - 3 AU - YUKA MURAMATSU-MAEKAWA AU - KYOJIRO KAWAKAMI AU - YASUNORI FUJITA AU - MANABU TAKAI AU - DAIKI KATO AU - KEITA NAKANE AU - TAKU KATO AU - TOMOHIRO TSUCHIYA AU - TAKUYA KOIE AU - YURI MIURA AU - MASAFUMI ITO AU - KOSUKE MIZUTANI Y1 - 2021/05/01 UR - http://cgp.iiarjournals.org/content/18/3/253.abstract N2 - Aim: To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). Materials and Methods: EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. Results: Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. Conclusion: These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC. ER -