TY - JOUR T1 - A Comprehensive Method for Detecting Fusion Genes in Paediatric Brain Tumours JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 343 LP - 348 DO - 10.21873/cgp.20093 VL - 15 IS - 4 AU - AKIHIDE KONDO AU - YUZABURO SHIMIZU AU - SATOSHI ADACHI AU - IKUKO OGINO AU - MARIO SUZUKI AU - OSAMU AKIYAMA AU - HAJIME ARAI Y1 - 2018/07/01 UR - http://cgp.iiarjournals.org/content/15/4/343.abstract N2 - Background: Fusion genes driving tumourigenesis have drawn the attention of researchers and oncologists. Despite the importance of such molecular alterations, there are no comprehensive reproducible methods for detecting fusion genes. Materials and Methods: Nineteen paediatric brain tumours of five types, namely pilocytic astrocytoma, oligodendroglioma, anaplastic astrocytoma, glioblastoma and, ganglioglioma, were examined to detect fusion genes using a pyrosequencing-based method following RNA isolation, cDNA synthesis and real-time polymerase chain reaction. Results: Our method successfully detected KIAA1549–v-raf murine sarcoma viral oncogene homolog B1 (BRAF) fusion in 14 out of 19 patients suffering from five types of paediatric brain tumours providing information on fusion breakpoints within 2 h. Conclusion: A comprehensive method for detecting fusion genes in paediatric brain tumours was evaluated. This method identified KIAA1549–BRAF fusion variants quickly. Our results may help researchers interested in the role of fusion genes in tumourigenesis. ER -